In Summary, My research interests are making better diagnostic tools, vaccines and Therapeutics using advanced Molecular Molecular Biology Techniques -Bioinformatics and Computational Biology. I have a background in Biochemistry (Bsc), Molecular Biology and Bioinformatics (Msc-Ongoing).  I have experience in Microbiology, Biochemistry, Molecular Biology, Biotechnology, Molecular Biology, Biotechnology, Immunology and Science Communication – Science writing, web development , Social Media & Photo/Videography.

I am looking for a Phd position in bioinformatics and/or Computational Biology. 

I am a Jill of all Trades but and a master of one – Investigation. 10 Years ago, I penned a list of 20 professions I eyed. The common things were the non-routine and research aspects. Today, I am still discovering what Profession suits my abilities. 

My first day in high school, I wasn’t sure what Technical subject to go for out of the 8, however I was sure that Business Studies would be number 8. 10 years later I have been involved in business management, development and Idea Criticism more than I thought I would ever be.

My love for Design, Maths, Physics and Computer Studies and my drawing Talent made me think I would end up in an Architectural Profession but the Kenyan Government’s algorithm chose Biochemistry due to my good Biology and Chemistry grades. Now all this have been packaged into Bioinformatics and Computational Biology. 

Currently, I am Masters student in JKUAT and an ILRI MSc Fellow working on project (Identification of virulence factors as novel vaccine targets for contagious bovine pleuropneumonia by whole genome saturated mutagenesis) incorporating Molecular Biology and Bioinformatics. As an African, I believe drugs, diagnostics and vaccines that suit our infrastructure and culture are a route to better interventions in Plants, animals and People. 

In this self-discovery journey, I believe there is more to learn about the world and myself. 

For details: Click on the Tabs below.

Degrees

2019-Present

Masters of Science in Molecular Biology and Bioinformatics

Jomo Kenyatta University of Agriculture and Technology_Website

I just finished my coursework and currently looking for a project preferably in diagnostics.

2014-2018

Bachelor of Science in Biochemistry

Technical University of Kenya_Website

Coursework, Projects, 1 internal Attachment, and 2 Internships.

Certificates

March 2020

Good Clinical Practice

IAVI – International Aids Vaccine Institute

March 2019

Fundamentals of Digital Marketing

Open University with Google_Website

2010-2013

KCSE- Kenya Certificate of Secondary Education

State House Girls High School_Website

A- (77 Points) for All Sciences, Geography and Computer Studies

2002-2009

KCPE- Kenya Certificate of Primary Education

Roysambu Primary School_Post

377/500.

Msc Graduate Fellow

April 2021- March 2022

ILRI-  International livestock Research Institute

Science Writer

March 2020- March 2021

Science Media Africa– Science for Everyone

Science Cast – Bridging the Gap Between scientists and the Public : (Company Sold)

Laboratory Intern

May 2016- March 2020

KAVI Institute of Clinical Research – PBMC Harvesting and Molecular Lab.

ICRAF – Genebank (Molecular and Seed Labs)

UNITID – HIVDR and TB Diagnostics

KEPHIS– HQ, Muguga and JKUAT Stations

USIU – School of Pharmacy and Health Sciences (Biochemistry and Microbiology Lab)

Science Communicator

May – October 2020

ISAAA  

ABBC 2019 Conference

Social Media Management

Website QA

CIP

Social media Strategy

Blog and Report Writing

Photography and Editing

 

Project Summary

Current Project 

Identification of virulence factors as novel vaccine targets for contagious bovine pleuropneumonia by whole genome saturated mutagenesis

There is an urgent need for improved vaccines for contagious bovine pleuropneumonia (CBPP). CBPP is one of the most infectious and highly contagious diseases of cattle in Africa accounting for more than $38.8 million annual loss in cattle productivity in 12 endemic countries in sub-Saharan Africa. Currently, control of CBPP relies on a live vaccine of limited efficacy and occasional severe side effects. Achieving breakthroughs in developing effective tools, including vaccines, for CBPP intervention requires deeper insights into host-bacteria interactions.

Like other diseases caused by mycoplasma, CBPP is characterized by immunopathology, i.e. dysregulation of the host’s immune response. In vitro data indicates that this dysregulation begins at the very early stages of infection, at the initial site of bacteria-host cell interaction in the lung. We aim towards identifying vaccine candidates that can disrupt the bacteria-induced dysregulation of the host’s immune response.

To pump-prime this objective, we propose to generate a random mutant library of the bacteria which can be used to identify bacterial genes that play an important role in the initial bacteria-host cell encounters and the dysregulation of the host’s immune response. Within the scope of the initial project, we will use the mutant library in established in vitro assays to screen for bacteria that fail to induce the typical response (compared to the wild type bacteria). This will allow a deeper understanding of host-pathogen interactions, and the identified antigens will be tested in future in vivo experiments for their suitability as vaccine candidates.

 

Original text

 

In-Vitro Adsorption activity of Activated Charcoal on Coliform Bacteria 

Activated charcoal has been used before to adsorb different molecules including toxins and drug overdoses. Little research has been done on its ability to adsorb microorganisms. The aim of the study was to examine the adsorption characteristic of activated charcoal on gram-positive and gram-negative bacteria using Staphylococcus aureus and Shigella spp . respectively as representatives. The other objective was to find out the effectiveness of activated charcoal on adsorbing bacteria. The study showed that it was effective on gram-negative bacteria more than the gram-negative bacteria. In all, it was more effective with the treatment of 10mg activated charcoal as the effect was dose-dependent. This shows that activated charcoal can be used to remove bacteria from the gastrointestinal tract thus treating diarrheal diseases as previously thought.

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Coenzyme Q10 nullified khat-induced hepatotoxicity, nephrotoxicity and inflammation in a mouse model

I briefly supported the project where I administered mice with Khat after crushing the Khat – Dr. Kennedy Chepukosi. I gained skills in animal handling and how to use a mice a model.

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I briefly supported Lamp Optimization for a Field-based Diagnosis of a sweet potato viral Disease Under Bramwel Wanjala

I also briefly supported a micorbial-base project where investigation of diesel as a food source for bacteria was being done.

Researching on ‘Data protection Act an its effect on research in Kenya’ article under Dr. Caleb Kibet

  1. Molecular Biology of Bacteria, Viruses, Fungi, and Nematodes: PCR, Gel, Quantification, Sanger and Nanopore sequencing
  2. Immunological techniques: PBMC Harvesting, ELISA’s including Multiplex using Luminex and Flow Cytometry
  3. Culture techniques; Bacterial, Plant Tissue, cell, viral and Fungal.
  4. Science Communication
  5. Informal Writing: Blogging
  6. Website Design (WordPress)
  7. Digital Marketing: Social Media, Email, and SEO.
  8. Microsoft applications
  9. Photography, Video editing, and graphic design

Kemri-Human DNA Identification (Forensics) Lab

Beca-Ilri Lab

Icipe Insects Lab

Kenya Veterinary production institute Labs

Kenya Marine fisheries Institute Labs

Government Chemist Mombasa Labs

Black Women in Computational Biology_Website

Bioinformatics Hub of Kenya_Website

International Veterinary Vaccinology Network_Website

Website Development

Bioinformatics Hub of Kenya Initiative

Dr. Mercy Akinyi

Social Media Marketing

Kuza Initiative

WordCamp Nairobi

Imba Kwa Akili

English

Swahili

R

German

Python

HTML

CSS